Deteksi Resistensi Isolat Bakteri Salmonella sp. dari Feses Segar Ayam Petelur (Gallus gallus domesticus) terhadap Antibiotik

Widia Restu; Candra Dwi Atma; Yeti Kurniasih; Kholik Kholik

doi:10.36312/biocaster.v6i3.1199

Authors

Widia Restu Program Studi Pendidikan Dokter Hewan, Fakultas Kedokteran Hewan, Universitas Pendidikan Mandalika, Jalan Pemuda Nomor 59A, Mataram, Nusa Tenggara Barat 83125, Indonesia
Candra Dwi Atma Departemen Mikrobiologi dan Parasitologi Veteriner, Fakultas Kedokteran Hewan, Universitas Pendidikan Mandalika, Jalan Pemuda Nomor 59A, Mataram, Nusa Tenggara Barat 83125, Indonesia
Yeti Kurniasih Departemen Kedokteran Veteriner, Fakultas Kedokteran Hewan, Universitas Pendidikan Mandalika, Jalan Pemuda Nomor 59A, Mataram, Nusa Tenggara Barat 83125, Indonesia
Kholik Kholik Departemen Kesehatan Masyarakat Veteriner, Fakultas Kedokteran Hewan, Universitas Pendidikan Mandalika, Jalan Pemuda Nomor 59A, Mataram, Nusa Tenggara Barat 83125, Indonesia

DOI:

https://doi.org/10.36312/biocaster.v6i3.1199

Keywords:

Antibiotics, Laying Hens, Disc Diffusion, Antibiotic Resistance, Salmonella sp.

Abstract

Antibiotic resistance is a global problem that connects human and animal health. This resistance occurs when bacteria acquire genes that allow them to survive when exposed to antibiotics. Wanasaba District, East Lombok is one of the districts that has a large population of laying hens, one of the infectious diseases that often attacks poultry and is treated using antibiotics is salmonella sp. This study aims to detect the resistance of Salmonella sp. bacterial isolates derived from fresh feces of laying hens (Gallus gallus domesticus) to antibiotics. This study is a laboratory experimental study conducted in November 2025 with a total of 3 samples of fresh feces of laying hens taken using a purposive sampling method. Bacterial isolation was carried out using Xylose Lysine Deoxycholate (XLD) agar media, then identified using biochemical tests to confirm the presence of Salmonella sp. Antibiotic resistance tests were carried out using the Kirby-Bauer disc diffusion method using 30 μg tetracycline antibiotics, 10 μg gentamicin, and 5 μg enrofloxacin. The results showed that all Salmonella sp. isolates from the three samples demonstrated 100% sensitivity to the three types of antibiotics tested. These results indicate that Salmonella sp. isolates in laying hens at the study site have not yet developed resistance to the antibiotics used. However, monitoring antibiotic use and implementing good farm management are still necessary to prevent the emergence of bacterial resistance in the future.

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References

Annisa, U., Sudarwanto, M., Soviana, S., & Pisestyani, H. (2020). Keberadaan Salmonella Sp. pada Susu Olahan Asal Kedai Susu di Sekitar Permukiman Mahasiswa Institut Pertanian Bogor. Jurnal Kajian Veteriner, 8(1), 34-42. https://doi.org/10.35508/jkv.v8i1.2229

April, B. R., Agustin, A. L. D., Atma, C. D., & Tirtasari, K. (2022). Detection of Antibiotic Resistance Salmonella Sp Isolated from Layer Chicken Farm in Sesaot West Lombok. Media Kedokteran Hewan, 33(1), 18–25. https://doi.org/10.20473/mkh.v33i1.2022.18-25

BPS. (2024). Statistik Indonesia 2024. Jakarta: Badan Pusat Statistik Republik Indonesia.

Brunton, L., Knollmann, B., & Hilal-Dandan, R. (2017). Goodman and Gilman's The Pharmacological Basis of Therapeutics, 13th Edition. New York: McGraw Hill Professional.

CLSI. (2017). Performance Standards for Antimicrobial Susceptibility Testing (27th ed.). Pennsylvania: Clinical and Laboratory Standards Institute.

CLSI. (2024). Performance Standards for Antimicrobial Susceptibility Testing (34th ed.). Pennsylvania: Clinical and Laboratory Standards Institute.

Erina, E., Abrar, M., Suyoto, B. A., Dewi, M., Darmawi, D., Darniati, D., & Bakri, M. (2017). Isolation and Identification of Salmonella sp. in Spleen of Male Layer Chicken in Sibreh Farms, Aceh Besar. Jurnal Medika Veterinaria, 11(1), 26–34. https://doi.org/10.21157/j.med.vet..v11i1.3986

FAO. (2021). The State of Food and Agriculture 2021: Making Agrifood Systems More Resilient to Shocks and Stresses. Rome: Food and Agriculture Organization.

FDA. (2021). Novel Drug Approvals for 2021. Maryland: Food and Drug Administration.

Martin, J. E., & Hurd, A. J. (1987). Scattering from Fractals. Journal of Applied Crystallography, 20(1), 61–78. https://doi.org/10.1107/S0021889887087107

Martsiningsih, M. A., Suyana, Noviani, A., Rahmawati, U., Sujono, & Astuti, F. D. (2023). Pengaruh Waktu Inkubasi terhadap Diameter Zona Hambat Antibiotik pada Uji Sensitivitas Bakteri Klebsiella pneumonia. Meditory : The Journal of Medical Laboratory, 11(1), 1-8. https://doi.org/10.33992/meditory.v11i1.2361

Notoatmodjo, S. (2018). Metodologi Penelitian Kesehatan. Jakarta: Rineka Cipta.

Ramdani, F. A., Riwu, K. H. P., Kholik, Mbura, Y. V. H., & Zakarias, H. V. (2024). Pattern of Antibiotic Resistance in Salmonella sp. Bacteria Contaminating Fresh Faeces of Laying Hens in Kediri District, West Lombok Regency. Biota : Jurnal Ilmiah Ilmu-ilmu Hayati, 9(2), 145–154. https://doi.org/10.24002/biota.v9i2.7814

Spellberg, B., Bartlett, J. G., & Gilbert, D. N. (2013). The Future of Antibiotics and Resistance. New England Journal of Medicine, 368(4), 299–302. https://doi.org/10.1056/NEJMp1215093

Sugiyono. (2019). Metode Penelitian Kuantitatif, Kualitatif, dan R&D. Bandung: CV Alfabeta.

Thaha, A. H. (2017). Gambaran Klinis dan Prevalensi Salmonellosis pada Ayam Ras Petelur di Desa Tanete Kec. Maritenggae Kabupaten Sidrap. Jurnal Ilmu dan Industri Peternakan, 3(1), 160-168. https://doi.org/10.24252/jiip.v3i1.3926

Untari, M. F. A., Widayani, A., & Indriani, R. (2021). Analisis Bibliometrik Perkembangan Penelitian Literasi Digital di Indonesia. Jurnal Pustakawan Indonesia, 21(2), 91–106. https://doi.org/10.29244/jpi.21.2.91-106

WHO. (2015). Global Action Plan on Antimicrobial Resistance. Jenewa: World Health Organization.

WOAH. (2022). The Humans Behind Animal Health. Paris: World Organisation for Animal Health.

Zhang, X. (2024). Residue Elimination Patterns and Determination of the Withdrawal Times of Seven Antibiotics in Eggs of Taihang Chickens. Animals, 14(4), 1-12. https://doi.org/10.3390/ani14040567