Identifikasi Bakteri dan Penentuan Jumlah Koloni pada Masker Sekali Pakai Berdasarkan Lama Waktu Pemakaian

Ni Putu Widayanti; Ayu Saka Laksmita W; Desak Putu Risky Vidika Apriyanthi

doi:10.36312/biocaster.v6i3.1372

Authors

Ni Putu Widayanti Program Studi Teknologi Laboratorium Medik, Fakultas Ilmu-ilmu Kesehatan, Universitas Bali Internasional, Jalan Seroja Gang Jeruk Nomor 9A, Denpasar, Bali 80234, Indonesia
Ayu Saka Laksmita W Program Studi Teknologi Laboratorium Medik, Fakultas Ilmu-ilmu Kesehatan, Universitas Bali Internasional, Jalan Seroja Gang Jeruk Nomor 9A, Denpasar, Bali 80234, Indonesia
Desak Putu Risky Vidika Apriyanthi Program Studi Teknologi Laboratorium Medik, Fakultas Ilmu-ilmu Kesehatan, Universitas Bali Internasional, Jalan Seroja Gang Jeruk Nomor 9A, Denpasar, Bali 80234, Indonesia

DOI:

https://doi.org/10.36312/biocaster.v6i3.1372

Keywords:

Total Plate Count, Bacillus sp., Bacteria, Disposable Masks, Staphylococcus sp.

Abstract

Prolonged use of disposable masks can increase bacterial accumulation due to exposure to droplets, aerosols, and respiratory humidity. This study aims to identify the types of bacteria and determine the number of bacterial colonies on disposable masks based on the duration of use by educational laboratory staff at Bali International University. This study used an analytical observational design with a cross-sectional approach. Samples consisted of 15 disposable masks grouped by duration of use: 3 hours, 6 hours, and 9 hours, with 5 samples each. Examination was carried out through bacterial culture, microbiological identification, and total plate count calculation. The results showed that the number of bacterial colonies increased with increasing duration of mask use. The average total plate count at 3 hours of use was 31.00 ± 9.62 CFU/g, increased to 138.00 ± 33.47 CFU/g at 6 hours, and reached 646.00 ± 122.39 CFU/g at 9 hours. The dominant bacteria found were Bacillus sp. and Staphylococcus sp., with Bacillus sp. dominating across all sample groups. This study concluded that the longer disposable masks were used, the higher the number of bacterial colonies accumulated. Regular mask replacement every 4–6 hours is recommended to maintain hygiene and safety in the laboratory.

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References

Ahadi, G. D., & Zain, N. N. L. E. (2023). The Simulation Study of Normality Test Using Kolmogorov-Smirnov, Anderson-Darling, and Shapiro-Wilk. EIGEN Mathematics Journal, 6(1), 11–19. https://doi.org/10.29303/emj.v6i1.131

Delanghe, L., Cauwenberghs, E., Spacova, I., De Boeck, I., Van Beeck, W., Pepermans, K., Claeys, S., Jorissen, M., & Lebeer, S. (2021). Cotton and Surgical Face Masks in Community Settings: Bacterial Contamination and Face Mask Hygiene. Frontiers in Medicine, 8(1), 1–12. https://doi.org/10.3389/fmed.2021.732047

Ding, G., Li, G., Liu, M., Sun, P., Ren, D., Zhao, Y., Gao, T., Yang, G., Fang, Y., & Li, W. (2023). Bacterial Contamination of Medical Face Mask Wearing Duration and the Optimal Wearing Time. Frontiers in Cellular and Infection Microbiology, 13(1), 1-8. https://doi.org/10.3389/fcimb.2023.1231248

Hafezi, A., & Khamar, Z. (2024). The Method and Analysis of Some Biochemical Tests Commonly Used for Microbial Identification: A Review. Comprehensive Health and Biomedical Studies, 3(2), 1-13. https://doi.org/10.5812/chbs-160199

Indrayati, S., Utami, P. R., & Cahyadi, R. (2023). Identifikasi Bakteri pada Masker Medis setelah 4 Jam dan 8 Jam Pemakaian. Bioma : Jurnal Biologi Makassar, 8(1), 1–8. https://doi.org/10.20956/bioma.v8i1.24061

Kisielinski, K., Wojtasik, B., Zalewska, A., Livermore, D. M., & Jurczak-Kurek, A. (2024). The Bacterial Burden of Worn Face Masks - Observational Research and Literature Review. Frontiers in Public Health, 12(1), 1–16. https://doi.org/10.3389/fpubh.2024.1460981

Prada, A. F., Distler, A., Cheng, S., Scott, J. W., Chamorro, L. P., Subramanian, G., Verma, V., & Turner, A. (2023). Disposable Face Masks: A Direct Source for Inhalation of Microplastics. Environmental Science & Technology, 57(3), 1234–1242. https://doi.org/10.1021/acs.est.2c06998

Soo, J. C., Monaghan, K., Lee, T., Kashon, M., & Harper, M. (2024). Microplastic Release from Disposable Face Masks Associated with Prolonged Usage. Science of the Total Environment, 912(1), 1-20. https://doi.org/10.1016/j.scitotenv.2023.169428

Yang, Y., Qu, L., Li, Y., Fang, X., & Zhang, R. (2022). Advances in the Human Skin Microbiota and Its Roles in Cutaneous Diseases. Microbial Cell Factories, 21(1), 1-14. https://doi.org/10.1186/s12934-022-01901-6

Yousefimashouf, R., Hamedani, S., & Bahmani, M. (2023). Evaluation of Bacterial Contamination on Medical Masks after Prolonged Use among Healthcare Workers. Journal of Preventive Medicine and Hygiene, 64(2), 210–216. https://doi.org/10.15167/2421-4248/jpmh2023.64.2.2703

Zhang, G., Ferguson, M., & Blodgett, R. J. (2026). Bacteriological Analytical Manual Chapter 3: Aerobic Plate Count. Maryland: Food and Drug Administration.