Pengaruh Konsumsi Daging Wagyu dan Daging Meltique terhadap Kadar Kolesterol Mencit (Mus musculus) Jantan

Authors

  • Dwi Kurnia Wijaya Departemen Kedokteran Dasar Veteriner, Fakultas Kedokteran Hewan, Universitas Pendidikan Mandalika, Jalan Pemuda Nomor 59A, Mataram, Nusa Tenggara Barat 83125, Indonesia
  • Katty Hendriana Priscillia Riwu Departemen Kesehatan Veteriner, Fakultas Kedokteran Hewan, Universitas Pendidikan Mandalika, Jalan Pemuda Nomor 59A, Mataram, Nusa Tenggara Barat 83125, Indonesia
  • Iwan Doddy Dharmawibawa Departemen Mikrobiologi dan Parasitologi Veteriner, Fakultas Kedokteran Hewan, Universitas Pendidikan Mandalika, Jalan Pemuda Nomor 59A, Mataram, Nusa Tenggara Barat 83125, Indonesia
  • Alifianita Anake Yansri Departemen Ilmu Klinik Veteriner, Fakultas Kedokteran Hewan, Universitas Pendidikan Mandalika, Jalan Pemuda Nomor 59A, Mataram, Nusa Tenggara Barat 83125, Indonesia
  • Candra Dwi Atma Departemen Mikrobiologi dan Parasitologi Veteriner, Fakultas Kedokteran Hewan, Universitas Pendidikan Mandalika, Jalan Pemuda Nomor 59A, Mataram, Nusa Tenggara Barat 83125, Indonesia
  • Fitrah Ramdani Departemen Ilmu Klinik Veteriner, Fakultas Kedokteran Hewan, Universitas Pendidikan Mandalika, Jalan Pemuda Nomor 59A, Mataram, Nusa Tenggara Barat 83125, Indonesia
  • Muhammad Munawaroh Departemen Kedokteran Dasar Veteriner, Fakultas Kedokteran Hewan, Universitas Pendidikan Mandalika, Jalan Pemuda Nomor 59A, Mataram, Nusa Tenggara Barat 83125, Indonesia
  • Muh. Sofyan Departemen Kedokteran Dasar Veteriner, Fakultas Kedokteran Hewan, Universitas Pendidikan Mandalika, Jalan Pemuda Nomor 59A, Mataram, Nusa Tenggara Barat 83125, Indonesia

DOI:

https://doi.org/10.36312/biocaster.v6i3.1598

Keywords:

Cholesterol, Wagyu and Meltique Beef Consumption, Male Mice

Abstract

Public demand for premium meat is rising, driving increased consumption of Wagyu and Meltique beef. This study aimed to determine the effect of consuming Wagyu and Meltique beef on cholesterol levels in male mice (Mus musculus). Male mice were selected due to their physiological, anatomical, nutritional, pathological, and metabolic similarities to humans, as well as their common use in cholesterol research. A total of 25 male mice were used in this study, which was conducted from March to April 2026 at the Veterinary Medicine Laboratory (RSHP) of Mandalika University of Education (UNDIKMA). Cholesterol levels were assessed by collecting blood from the tail vein of treated mice on days 7, 14, 21, and 28 using heparinized capillary tubes. Cholesterol levels were measured periodically and analyzed using a one-way ANOVA test. The results indicated that the consumption of both Wagyu and Meltique beef was associated with increased cholesterol levels in male mice. In the Wagyu group, levels rose from 81.22 mg/dL in the first week to 102.00 mg/dL in the fourth week, while the Meltique group saw an increase from 98.33 mg/dL to 123.22 mg/dL over the same period. The study concluded that the consumption of Wagyu and Meltique beef had a significant effect on increasing cholesterol levels in male mice (Mus musculus).

Downloads

Download data is not yet available.

References

Ahmad Najib, M. S., Elias, M. H., Juliana, N., Sheikh Abdul Kadir, S. H., Ibrahim, E., & Abdul Hamid, N. (2025). The Effects of High-Fat Diet on the Molecular Pathways in Cardiac Tissue: A Systematic Review of In Vivo Rodent Studies and Integrated Bioinformatic Analysis. Biomedicines, 13(9), 1-14. https://doi.org/10.3390/biomedicines13092071

Chen, X., Zhou, Y., Yang, J., Yang, R., Xue, S., Wang, Q., & Niu, W. (2025). Animal Model Screening For Hyperlipidemic ICR Mice. International Journal of Molecular Sciences, 26(5), 2142-2151. https://doi.org/10.3390/ijms26052142

Dairoh, D., Sutikno, S., Ishak, A. B. L., Priyanto, R., Sumantri, C., Ulum, M. F., & Jakaria, J. (2024). Fatty Acid Profiling of Bali and Wagyu Cattle Using Principal Component Analysis. Buletin Peternakan, 48(1), 64–69. https://doi.org/10.21059/buletinpeternak.v48i1.86454

De Smet, S., & Van Hecke, T. (2024). Meat Products in Human Nutrition and Health: About Hazards and Risks. Meat Science, 218(1), 1-20. https://doi.org/10.1016/j.meatsci.2024.109628

Federer, W. (1963). Experimental Design Theory and Aplication. Oxford: Oxford and Lbh Publish Hinco.

Ferguson, N. M., Laydon, D., Nedjati-Gilani, G., Imai, N., Ainslie, K., Baguelin, M., Bhatia, S., Boonyasiri, A., Cucunubá, Z. M., Cuomo-Dannenburg, G., Dighe, A., Dorigatti, I., Fu, H., Gaythorpe, K., Green, W., Hamlet, A., Hinsley, W., Okell, L., van Elsland, S. L., … Ghani, A. C. (2020). Report 9: Impact of Non-Pharmaceutical Interventions (NPIs) to Reduce COVID-19 Mortality and Healthcare Demand. London: Imperial College London.

Gotoh, T., & Joo, S. T. (2016). Characteristics and Health Benefit of Highly Marbled Wagyu and Hanwoo Beef. Korean Journal for Food Science of Animal Resources, 36(6), 709–718. https://doi.org/10.5851/kosfa.2016.36.6.709

Grundy, S. M., Stone, N. J., Bailey, A. L., Beam, C., Birtcher, K. K., Blumenthal, R. S., Braun, L. T., de Ferranti, S., Faiella-Tommasino, J., Forman, D. E., Goldberg, R., Heidenreich, P. A., Hlatky, M. A., Jones, D. W., Lloyd-Jones, D., Lopez-Pajares, N., Ndumele, C. E., Orringer, C. E., Peralta, C. A., Saseen, J. J., Smith Jr, S. C. S., Sperling, L., Virani, S. S., & Yeboah, J. (2019). 2018 AHA/ACC/AACVPR/AAPA/ABC/ACPM/ADA/AGS/ APhA/ASPC/NLA/PCNA Guideline on the Management of Blood Cholesterol: A Report of the American College of Cardiology/American Heart Association Task Force on Clinical Practice Guidelines. Circulation, 139(25), 1082–1143. https://doi.org/10.1161/CIR.0000000000000625

Kim, S. H., Yoon, H. J., & Kwon, Y. H. (2023). Increased Cholesterol Uptake is Associated with the Altered Gene Expression in White Adipose Tissue of ApoE-/- Mice Fed a High-Fat High-Cholesterol Diet. Biochemical and Biophysical Research Communications, 676(1), 109–114. https://doi.org/10.1016/j.bbrc.2023.07.043

Komara, F. R., Nurhayati, N., & Susanti, L. (2024). Karakteristik Nutrisi dan Marbling pada Daging Meltique Berbasis Injeksi Lemak Nabati. Jurnal Ilmu dan Teknologi Peternakan Indonesia, 15(1), 21–28.

Komatsu, K. J., Avolio, M. L., Lemoine, N. P., Isbell, F., Grman, E., Houseman, G. R., Koerner, S. E., Johnson, D. S., Wilcox, K. R., Alatalo, J. M., Anderson, J. P., Aerts, R., Baer, S. G., Baldwin, A. H., Bates, J., Beierkuhnlein, C., Belote, R. T., Blair, J. M. G., Bloor, J. M. G., … Zhang, Y. (2019). Global Change Effects on Plant Communities are Magnified by Time and the Number of Global Change Factors Imposed. Proceedings of the National Academy of Sciences, 116(36), 17867–17873. https://doi.org/10.1073/pnas.1819027116

Mariana, E., & Rahmadi, A., & Syahrumsyah. (2020). Pengaruh Pemberian Cuka Mandai terhadap Kadar Kolesterol Total, Lipoprotein dan Trigliserida pada Mencit (Mus musculus) dengan Induksi Kuning Telur. Journal of Tropical AgriFood, 3(2), 45-52. http://dx.doi.org/10.35941/jtaf.2.1.2020.3918.45-52

Mosquera, J. V., Auguste, G., Wong, D., Turner, A. W., Hodonsky, C. J., Alvarez-Yela, A. C., Song, Y., Cheng, Q., Lino Cardenas, C. L., Theofilatos, K., Bos, M., Kavousi, M., Peyser, P. A., Mayr, M., Kovacic, J. C., Björkegren, J. L. M., Malhotra, R., Stukenberg, P. T., Finn, A. V., van der Laan, S. W., Zang, C., Sheffield, N. C., & Miller, C. L. (2023). Integrative Single-Cell Meta-Analysis Reveals Disease-Relevant Vascular Cell States and Markers in Human Atherosclerosis. Cell reports, 42(11), 1-51. https://doi.org/10.1016/j.celrep.2023.113380

Muhammad, I. (2014). Penilaian Cara Mengajar Menggunakan Rancangan Acak Lengkap (Studi Kasus: Cara Mengajar Dosen Jurusan Statistika UNDIP). Thesis. Faculty of Science and Mathematics Universitas Diponegoro.

Prabhu, V., Ashok, L., Kamath, V. G., Puranik, A., Padickaparambil, S., Rebello, N. S., Arhanthabailu, P., & Chandrasekaran, V. (2025). Evaluating the Effectiveness of a Literacy Module - A Randomized Controlled Trial among School Teachers. Clinical Epidemiology and Global Health, 32(1), 1-9. https://doi.org/10.1016/j.cegh.2025.101946

Rahayuningrum. (2021). Gambaran Histologis Hati dan Keterkaitannya dengan Kadar Kolesterol Total pada Mencit Swiss Webster Model Hiperkolesterolemia. Thesis. Universitas Pendidikan Indonesia.

Yudhistira, M., Apriantini, A., & Wulandari, Z. (2025). Tenderness Level and Sensory Analysis of Buffalo Meltique Meat Injected with Various Oils and Papain. Jurnal Ilmu Ternak Universitas Padjadjaran, 25(3), 216-223, https://doi.org/10.24198/jit.v25i3.66696

Zhang, Y., Li, X., Cirillo, P. M., & Wang, Y. (2020). High-Fat Diet-Induced Metabolic Disorder and Altered Gut Microbiota in Mice. Journal of Nutritional Biochemistry, 77(1), 1-20. https://doi.org/10.1016/j.jnutbio.2019.108319

Downloads

Published

2026-07-10

How to Cite

Wijaya, D. K., Riwu, K. H. P., Dharmawibawa, I. D., Yansri, A. A., Atma, C. D., Ramdani, F., Munawaroh, M., & Sofyan, M. (2026). Pengaruh Konsumsi Daging Wagyu dan Daging Meltique terhadap Kadar Kolesterol Mencit (Mus musculus) Jantan. Biocaster : Jurnal Kajian Biologi, 6(3), 1432–1442. https://doi.org/10.36312/biocaster.v6i3.1598