Ekspresi Gen GPx-3 pada Organ Otak Tikus Putih (Rattus norvegicus) yang Terpapar Stres Oksidatif Gandaria (Bouea macrophylla Griffith)

Dewi Syukrilla; Zahratul Idami; Nurlian Augustin Ningrum; Kartika Manalu

doi:10.36312/biocaster.v6i4.1712

Authors

Dewi Syukrilla Program Studi Pendidikan Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sumatera Utara, Jalan Lapangan Golf Nomor 120, Deli Serdang, Sumatera Utara 20353, Indonesia
Zahratul Idami Program Studi Pendidikan Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sumatera Utara, Jalan Lapangan Golf Nomor 120, Deli Serdang, Sumatera Utara 20353, Indonesia
Nurlian Augustin Ningrum Program Studi Pendidikan Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sumatera Utara, Jalan Lapangan Golf Nomor 120, Deli Serdang, Sumatera Utara 20353, Indonesia
Kartika Manalu Program Studi Pendidikan Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sumatera Utara, Jalan Lapangan Golf Nomor 120, Deli Serdang, Sumatera Utara 20353, Indonesia

DOI:

https://doi.org/10.36312/biocaster.v6i4.1712

Keywords:

Bouea macrophylla, GPx-3, Carbon Tetrachloride (CCl₄), RT-PCR, Oxidative Stress

Abstract

Oxidative stress is one of the factors that can cause neuronal damage in the brain through an excessive increase in free radical production that exceeds the capacity of the antioxidant defense system. Glutathione Peroxidase-3 (GPx-3) is an endogenous antioxidant enzyme that plays an important role in maintaining redox balance and protecting cells from oxidative damage. This study aimed to analyze the expression of the GPx-3 gene in the brain tissue of white rats (Rattus norvegicus) experiencing oxidative stress induced by carbon tetrachloride (CCl₄), as well as to evaluate the effect of ethanol extract of gandaria fruit (Bouea macrophylla Griffith.) on changes in GPx-3 gene expression. This experimental study used five treatment groups consisting of a negative control group (KN), a positive control group (K+), and three groups treated with different doses of gandaria fruit ethanol extract, namely 100 mg/kg body weight (P1), 150 mg/kg body weight (P2), and 200 mg/kg body weight (P3). Gene expression analysis was performed using Reverse Transcription Polymerase Chain Reaction (RT-PCR), with GAPDH used as a housekeeping gene for data normalization. The results showed that the KN group had a fold change value of 1, while the K+ group exhibited an increased GPx-3 gene expression with a fold change value of 1.7 following CCl₄ induction. Administration of gandaria fruit ethanol extract resulted in variations in GPx-3 gene expression among the treatment groups, with fold change values of 1.56 ± 0.69 in P1, 2.05 ± 1.22 in P2, and 2.77 ± 1.17 in P3. Based on the fold change values, the 100 mg/kg body weight dose (P1) showed a tendency to produce GPx-3 expression levels closest to the normal condition compared with other treatment groups. The alteration of GPx-3 expression indicates an adaptive response of the antioxidant defense system to oxidative stress conditions, suggesting that gandaria fruit ethanol extract has potential as a natural antioxidant source in supporting cellular protection against oxidative damage.

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References

Handayani, S. O., & Sudesty, S. P. (2026). Strategi Antioksidan dalam Menekan Stress Oksidatif : Bukti Pre-Klinis dan Klinis. Jurnal Kesehatan Tambusai, 7(2), 5858–5867. https://doi.org/10.31004/jkt.v7i2.56709

Jayantie, D. D., Farida, Y., & Taurhesia, S. (2022). Aktivitas Antioksidan dan Inhibisi Enzim Tirosinase Ekstrak Etanol Buah Gandaria (Bouea macrophylla Griff.) secara In Vitro. Pharmacoscript, 5(1), 63–70. https://doi.org/10.36423/pharmacoscript.v5i1.856

Johra, F. T., Hossain, S., Jain, P., Bristy, A. T., Emran, T., Ahmed, R., & Reza, H. M. (2023). Amelioration of CCl₄-Induced Oxidative Stress and Hepatotoxicity by Ganoderma lucidum in Long Evans Rats. Scientific Reports, 13(1), 1-13. https://doi.org/10.1038/s41598-023-35228-y

Kuang, J., Yan, X., Genders, A. J., Granata, C., & Bishop, D. J. (2018). An Overview of Technical Considerations when Using Quantitative Real-Time PCR Analysis of Gene Expression in Human Exercise Research. PLoS ONE, 13(5), 1-27. https://doi.org/10.1371/journal.pone.0196438

Mohseni, G. K., Hosseini, S. A., Majdinasab, N., & Cheraghian, B. (2023). Effects of N-Acetylcysteine on Oxidative Stress Biomarkers, Depression, and Anxiety Symptoms in Patients with Multiple Sclerosis. Neuropsychopharmacology Reports, 43(3), 382–390. https://doi.org/10.1002/npr2.12360

Munawaroh, F., Hanif, A. A., Kemuning, A. R., Mutmainah, I., Rachmawati, Y., & Nurdin, N. M. (2024). Efek Asam Klorogenat pada Ekspresi mRNA IL-6 dan CD86 Lobus Frontal Tikus Model Diabetes Mellitus. Plexus Medical Journal, 3(4), 154–162. https://doi.org/10.20961/plexus.v3i4.1815

Pei, J., Pan, X., Wei, G., & Hua, Y. (2023). Research Progress of Glutathione Peroxidase Family (GPX) in Redox Regulation. Frontiers in Pharmacology, 14(1), 1-14. https://doi.org/10.3389/fphar.2023.1141414

Pratiwi, S., Durry, M. F., & Kairupan, C. (2016). Gambaran Histopatologik Hati Tikus Wistar yang Diberi Minuman Kopi Pascainduksi Karbon Tetraklorida (CCl₄). Jurnal e-Biomedik, 4(1), 1-6. https://doi.org/10.35790/ebm.4.1.2016.12206

Rudiana, T., Fitriyanti, F., & Adawiah, A. (2018). Aktivitas Antioksidan dari Batang Gandaria (Bouea macrophylla Griff.). EduChemia : Jurnal Kimia dan Pendidikan, 3(2), 195-205. https://doi.org/10.30870/educhemia.v3i2.3328

Rusli, R. N. M., Naomi, R., Yazid, M. D., Embong, H., Perumal, K., Othman, F., Jasni, A. S., Jumidil, S. H., Balan, S. S., Abidin, A. Z., Kadir, K. K. A., Bahari, H., & Zakaria, Z. A. (2023). Phytochemical Analysis and Toxicity Assessment of Bouea macrophylla Yoghurt. Toxins, 15(2), 1-18. https://doi.org/10.3390/toxins15020125

Sari, N., Kristanti, H., & Anggraeni, D. N. (2024). Tingkat Kemurnian Hasil Isolat DNA Mahasiswa STIKES Wira Husada Yogyakarta Berkaitan dengan Pola Makan. Healthy Tadulako Journal (Jurnal Kesehatan Tadulako), 10(4), 565–572. https://doi.org/10.22487/htj.v10i4.1313

Sattari, M., Amri, J., Shahaboddin, M. E., & Panahi, G. (2024). The Protective Effects of Fisetin in Metabolic Disorders: A Focus on Oxidative Stress. Journal of Diabetes & Metabolic Disorders, 23(2), 1753–1771. https://doi.org/10.1007/s40200-024-01502-7

Shrivastav, D., Dabla, P. K., Sharma, J., Viswas, A., & Mir, R. (2023). Insights on Antioxidant Therapeutic Strategies in Type 2 Diabetes Mellitus. World Journal of Diabetes, 14(6), 919–929. https://doi.org/10.4239/wjd.v14.i6.919

Sies, H., Berndt, C., & Jones, D. P. (2017). Oxidative Stress. Annual Review of Biochemistry, 86(1), 715–748. https://doi.org/10.1146/annurev-biochem-061516-045037

Yeo, B. T., Krienen, F. M., Chee, M. W., & Buckner, R. L. (2014). Estimates of Segregation and Overlap of Functional Connectivity Networks in the Human Cerebral Cortex. NeuroImage, 88(1), 212–227. https://doi.org/10.1016/j.neuroimage.2013.10.046

Zhang, N., Liao, H., Lin, Z., & Tang, Q. (2024). Insights into the Role of Glutathione Peroxidase 3 in Non-Neoplastic Diseases. Biomolecules, 14(6), 1-31. https://doi.org/10.3390/biom14060689