Uji Total Plate Count (TPC) dan Cemaran Bakteri Coliform serta Escherichia coli pada Minuman Es Teh yang Dijual di Kecamatan Tanjung Morawa, Deli Serdang

Hanifah Dzaky Fadhilah; Marlinda Nilan Sari Rangkuti

doi:10.36312/biocaster.v6i4.1736

Authors

Hanifah Dzaky Fadhilah Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Negeri Medan, Jalan William Iskandar Ps. V, Deli Serdang, Sumatera Utara 20221, Indonesia
Marlinda Nilan Sari Rangkuti Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Negeri Medan, Jalan William Iskandar Ps. V, Deli Serdang, Sumatera Utara 20221, Indonesia

DOI:

https://doi.org/10.36312/biocaster.v6i4.1736

Keywords:

Coliform, Ice Cubes, Iced Tea, Escherichia coli, Brewed Tea

Abstract

Iced tea is one of the beverages widely consumed by the public, but it has the potential to undergo microbiological contamination due to poor hygiene and sanitation practices and environmental conditions at the point of sale. This study aimed to determine the Total Plate Count (TPC), contamination by Coliform bacteria and Escherichia coli, and to identify potential sources of microbiological contamination in iced tea sold in Tanjung Morawa District. This study used a descriptive quantitative method involving Total Plate Count (TPC) examination, the Most Probable Number (MPN) method through presumptive and confirmatory tests, and identification of Escherichia coli using Eosin Methylene Blue Agar (EMBA) and Gram staining. The results showed that all brewed tea samples had TPC values ranging from 3.3×10³–9.2×10³ CFU/mL and did not meet the requirements of BPOM RI Regulation Number 13 of 2019, while all ice cube samples had TPC values ranging from 3.2×10³–5.7×10³ CFU/mL and still met the requirements of SNI 3839:2019. All brewed tea and ice cube samples were positive for Coliform, with MPN values at 37°C ranging from 43–460 MPN/100 mL and at 44°C ranging from 23–93 MPN/100 mL, indicating that they did not meet the microbiological requirements based on the Regulation of the Minister of Health of the Republic of Indonesia Number 492/Menkes/Per/IV/2010. Escherichia coli was detected in all brewed tea samples and in five of the eight ice cube samples. The results indicated that the brewed tea component was suspected to contribute more to microbiological contamination than the ice cube component. The hygiene of the vendors and environmental conditions at the points of sale also indicated factors that may support the occurrence of contamination. Therefore, improving the implementation of hygiene and sanitation practices during the preparation and serving of iced tea is necessary to reduce the risk of microbiological contamination.

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